Spata19 Inactivation as a Cause of Oligospermia

Background: Spermatogenesis associated 19 (Spata19) was introduced as a testis-specific gene that probably involved in spermatogenesis cell apoptosis. Therefore, this study aimed to investigate the effect of Spata19 inactivation on sperm count. Materials and Methods: We generated global knockout mice by CRISPR/Cas9 nickase technology. Disability validated three levels DNA, RNA, protein using PCR, RT-PCR, immunohistochemistry. Histological studies were performed for testis. Sperm characteristics also assessed with CASA software. Results: had 43 nucleotides deletion exon 4 gene. The presence absence confirmed normal mice, respectively. NMRI detected brain, heart, thymus semi-nested RT-PCR Leydig cells revealed decrease count mice. Also, parameters significantly reduced (P<0.05). Conclusion: These data indicate is cause oligospermia, its role could be beyond an adhesive molecule.